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Biotium
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Image Search Results
Journal: PLoS ONE
Article Title: Intestinal Tissues Induce an SNP Mutation in Pseudomonas aeruginosa That Enhances Its Virulence: Possible Role in Anastomotic Leak
doi: 10.1371/journal.pone.0044326
Figure Lengend Snippet: (A) IEC-18 cells infected with P1 and P2 for 3 hrs were analyzed for apoptosis and necrosis with Apoptic&Necrotic&Healthy Cells Quantification kit (Biotium, Inc.) using fluorescence microscope Axiovert 35 (Zeiss,Germany). FITC-Annexin V (apoptotic cells, green), EtD-III (necrotic cells, red). (B) Staining of nuclei with Hoechst 33342. (C) IEC-18 monolayers treated with antibody to ZO-1.
Article Snippet: Cell were incubated for 3 hrs at 37C°, 5% CO 2 , followed by analysis for apoptosis and necrosis using
Techniques: Infection, Fluorescence, Microscopy, Staining